ABSTRACT
Adult stem cells have great advantage in tissue reconstruction and regeneration. Transplantation of autologous stem cells into ischemic tissue is a novel therapeutic option for ischemic disorders. This review summarizes the potential role of adipose tissue-derived stromal cells (ADSC) on regenerative cell therapy for ischemic diseases. ADSC can be readily harvested and cultured; under specific condition, they can be induced to differentiate into adipocytes, bone, neurons, and endothelial cells. Moreover, ADSC can secrete a number of angiogenesis-related cytokines which might be suitable for regenerative cell therapy. It has also been reported that ADSC could differentiate into myocardiocytes. ADSC might be an important material for regenerative cell therapy in the near future, replacing bone marrow cells.
ABSTRACT
Objective: To establish a method for isolating adipose derived stem cells (ADSCs) from resected human subcutaneous adipose tissues. Methods: ADSCs were isolated, cultured, and expanded from human subcutaneous adipose tissues. Immuno-fluorescent staining of specific molecules, FACS and multi-lineage differentiation induction were used to characterize the obtained ADSCs. Results: ADSCs obtained in this study had the characteristics of stem cells and expressed specific molecules; they also possessed a multi-lineage differentiation potential,which was genetically stable. Conclusion: ADSCs can be isolated from human subcutaneous adipose tissues, which provides a novel and abundant seeding cells for tissue engineering.
ABSTRACT
Objective:To establish a method for isolating adipose derived stem cells(ADSCs)from resected human subcutaneous adipose tissues.Methods:ADSCs were isolated,cultured,and expanded from human subcutaneous adipose tissues.Immuno-fluorescent staining of specific molecules.FACS and multi-lineage differentiation induction were used to characterize the obtained ADSCs.Results:ADSCs obtained in this study had the characteristics of stem cells and expressed specific molecules;they also possessed a multi-lineage differentiation potential,which was genetically stable.Conclusion:ADSCs can be isolated from human subcutaneous adipose tissues,which provides a novel and abundant seeding cells for tissue engineering.